Abstract: Objective To observe the effect of compound Huang Gan in delaying chronic renal failure in rats after 5/6
nephrectomy and explore the possible mechanisms. Methods High-performance liquid chromatography was used to was used
identify the components of compound Huang Gan extract. Rat models of 5/6 nephrectomy received a 12-week treatment with
intragastric administration of Niaoduqing, Cozaar, or compound Huang Gan at low, moderate or high doses (n=10). After the
treatments, the rats were sacrificed for detecting Scr, BUN, Ucr and 24h UPr , pathological examination of the renal tissues, and
determination of FN, MCP-1, and ICAM-1 expression levels in the renal tissues using RT-PCR and immunohistochemistry.
Results The major chemical components of compound Huang Gan extract included glycyrrhizin (0.61%), paeonol (1.2%), aloe
emodin (0.72%), rhein (0.85%), emodin (0.87%), chrysophanol (0.79%) and physcion (0.8%). Treatment with compound Huang
Gan at low, moderate and high doses significantly reduced Scr, BUN, Ucr , Ccr and 24 h UPr levels (P<0.05), improved
interstitial fibrosis and glomerulosclerosis, and reduced FN and ICAM-1 expressions (P<0.05) in rats following nephrectomy.
Conclusion Compound Huang Gan can improve the renal function and lessen glomerulosclerosis and renal interstitial fibrosis
to delay the progression of chronic renal failure in rat models of 5/6 nephrectomy.