Abstract: Objective To investigate the correlation of the polymorphisms of adiponectin receptor 2 (AdipoR2) gene +33371Gln/
Arg and cytochromes P4502E1 gene Rsa I (CYP2E1-Rsa I) as well as smoking with nonalcoholic fatty liver disease (NAFLD).
Methods The polymorphisms of AdipoR2 gene + 33371Gln/Arg and CYP2E1-Rsa I were analyzed with PCR technique in
peripheral blood leukocytes from 750 NAFLD cases and 750 healthy subjects. Results The frequencies of AdipoR2 gene +
33371Gln/Arg (A/A) and CYP2E1-Rsa I (c2/c2 ) were 39.20% and 71.73% in NAFLD cases, respectively, significantly higher than
those in healthy subjects (21.07% and 43.07%, respectively, P<0.01). The risk of NAFLD increased significantly in subjects
carrying + 33371Gln/Arg (A/A) (OR=2.4156, 95% CI=1.8164-4.0725) and CYP2E1-Rsa I (c2/c2) (OR=3.3547, 95% CI=
1.9182-4.5057). Combined analysis of the polymorphisms showed that the percentage of +33371Gln/Arg (A/A)/ CYP2E1-Rsa I
(c2/c2) was 32. 67% in NAFLD cases, significantly higher than that in the healthy subjects (6.40%, P<0.01), and subjects carrying
both + 33371Gln/Arg (A/A) and CYP2E1-Rsa I (c2/c2) had a high risk of NAFLD (OR=9.9264, 95% CI=4.2928-12.4241). The
smoking rate was significantly higher in the case group than in the control group (OR=2.5919, 95% CI=1.4194-4. 9527, P<0.01),
and statistical analysis suggested an interaction between smoking and +33371Gln/Arg (A/A)/CYP2E1-Rsa I (c2/ c2) to increase
the risk of NAFLD (OR=34.6764, 95% CI=18.9076-61.5825). Conclusion + 33371Gln/Arg (A/A), CYP2E1-Rsa I (c2/c2 ) and
smoking are risk factors for NAFLD and coordinately contribute to the occurrence of NAFLD.