Abstract: Objective To construct a GFP/Puro double-labeled lentiviral expression vector for CK8 silencing and assess the
effects of CK8 silencing on cell apoptosis. Methods The siRNA sequences of CK8 were inserted into the lentiviral expression
vector GV248 and transfected into 293T cells with the packaging plasmids PMD and SPA. The lentivirus was collected at 24
and 36 h post-transfection. Flow cytometry was used to detect the virus titer and the positive cells were selected with
puromycin. The knockdown of CK8 was examined by Western blotting. The effect of CK8 down-regulation on cell apoptosis
induced by cisplatin was detected with Annexin V/PI staining. Results and Conclusion We successfully constructed CK8
interference lentiviral vector and obtained a stable cell line with CK8 knock-down that was sensitive to cisplatin-induced
apoptosis.