Abstract: Objective To investigate the influence of electroporation on the immunogenicity of the DNA vaccine pVAXtG250FcGB.
Methods The DNA vaccine pVAX-tG250FcGB was constructed by inserting the coding gene of tG250 fusion genes
into the expression vector pVAX. The DNA vaccine was delivered in BALB/c mouse by electroporation or intramuscular
injection, and the induced antigen specific immune responses were compared. Results The vaccine delivered by
electroporation and intramuscular injection both induced immune responses in BALB/c mouse, but electroporation produced
an obviously stronger effect than intramuscular injection. Conclusion Electroporation-mediated DNA vaccine delivery can
produce strong immune response in mice and is an effective means for studying the immunogenic effect of DNA vaccine
pVAX-tG250FcGB.