Abstract: Objective To analyze the transcription factor binding sites and methylation in the promoter region of UCA1 gene.
Methods UCA1 gene promoter was analyzed using CpG software to predict the CpG island. MatrixCatch and TFSEARCH
were used to predict the potential transcription factor binding sites in UCA1 gene core promoter. ChIP assay was used to
identify the transcription factors binding to UCA1 gene core promoter. Results UCA1 gene promoter contained no CpG island
and was therefore a typical tissue-specific gene. There were 4 transcription factors associated with human cancers in UCA1
gene core promoter, but only one of them interacted with UCA1 gene core promoter. Conclusion There is no CpG island in
UCA1 gene promoter region, and the transcription factor c-Myb can specifically bind to the core promoter.