Abstract: Objective To explore the method for the isolation, cultivation, and purification of adipose-derived stem cells
(ADSCs) and examine the oncogenesis and homing of ADSCs to the liver in vivo. Methods ADSCs were isolated from female
mice by digestion with 0.075% collagenase I and the morphology of the isolated cells was observed with examination of the
cell surface markers and cell cycle. BALB/c mice were injected with 1×106 ADSCs on the back to evaluate the oncogenesis of
ADSCs or with 1 × 106 ADSCs stained with 5, 6-carboxyfluorescein diacetate-succinimidyl ester (CFSE) via the tail vein to
examine the cell homing to the liver. Results The isolated ADSCs highly expressed CD29 and CD44 and were negative for
CD34, CD45, CD11b and CD14. Cell cycle distribution analysis showed cell percentages in G0/G1, S, and G2/M phases of
80.1%, 7.9%, and 12%, respectively. The ADSCs had a low immunogenicity and did not express CD40, CD80, CD86, MHCI,
MHCII or PDL-1. After stimulation with IFN-γ, the expression of CD40, CD80 and PDL-1 were up-regulated slightly in the
cells. Dorsal injection of the ADSCs did not result in any tumor formation within 1 month, and ADSCs injected via the tail vein
showed cell homing to the liver. Conclusion Murine ADSCs can be isolated and expanded effectively by collagenase digestion
and adherent culture. The isolated ADSCs can successfully reside in the liver after implantation, and thus may serve as a
promising candidate cell in stem cell therapy of liver diseases.