Abstract: Objective To apply pyrosequencing technique in the detection of the common pathogens in sepsis. Methods The
primers for amplification and sequencing in pyrosequencing were designed according to alignment of the bacterial 16S rRNA
sequence. Bacterial genomic DNA was extracted for pyrosequencing, and the pathogen species were determined according to
the sequencing data obtained. Results Pyrosequencing effectively yielded the sequencing data of the 28 bp sequences of the
pathogens and clearly distinguished the pathogen species of Streptococcus pyogenes, Streptococcus pneumonia, Escherichia coli,
Pseudomonas aeruginosa, Klebsiella pneumonia, Neisseria meningitides, and Salmonella, but failed to distinguish Staphylococcus
epidermidis from Staphylococcus aureus. Conclusion Pyrosequencing technique can effectively distinguish the common
pathogens in sepsis at the species level.