Abstract: Objective To observe the effect of 11R-VIVIT on lipopolysaccharide (LPS)-induced expression of urokinase-type
plasminogen activator receptor (uPAR) in podocytes. Methods A LPS-induced proteinuria mouse model and in vitro cultured
podocytes treated with LPS were both divided into control group, LPS group and LPS+ 11 R-VIVIT group. The mRNA and
protein expressions of uPAR in mouse kidney tissues and the podocytes were measured by real-time qPCR, laser scanning
confocal microscopy and Western blotting. Results Compared with LPS group, LPS+11 R-VIVIT group showed a significantly
lowered urine albumin/creatinine ratio (P<0.001) and markedly reduced mRNA and protein expressions of uPAR (PuPAR mRNA<
0.001; PuPAR=0.001). Conclusion 11R-VIVIT can ameliorate proteinuria probably by decreasing the expression of uPAR in
podocytes.