Abstract: Objective To investigate the effect of small interfering RNA-mediated receptor-interacting protein kinase 1 (RIP1)
knockdown on the sensitivity of human oral squamous carcinoma cells to to oxaliplatin (L-OHP)-induced apoptosis and
explore a new target for clinical treatment of oral squamous carcinoma. Methods The viability of human oral squamous
carcinoma cell line KB exposed to different concentrations (0, 0.25, 0.5, 1, 2, 4 μmol/L) of L-OHP were detected by MTT assay.
PI/Annexin V staining was used to observe cell apoptosis in naive KB cells, cell and transfected with pSH1Si-RIP1 or with the
empty plasmid. Western blotting was used to detect RIP1 expression in KB cells exposed to L-OHP and in cells transfected
with pSH1Si-RIP1. Results Exposure to L-OHP (1μmol/L) for 24, 48, 72 h resulted in KB cell survival rates of 67.66%, 55.17%,
and 41.34% , respectively, but the cell apoptosis rate was only 9.6% following a 24-h exposure. KB cells transfected with
pSH1Si-RIP1 showed an apoptotic rate of 9.4%, which increased to 29.1% following L-OHP exposure. RIP1 expression was first
up-regulated and then down-regulated in KB cells treated with L-OHP, and was significantly reduced after cell transfection
with pSH1Si-RIP1. Conclusion Suppression of RIP1 expression increases the apoptotic rate of human oral squamous
carcinoma cells, suggesting the potential of RIP1 as a new candidate target for clinical treatment of oral squamous carcinoma.