Abstract: Objective To observe the effect of TSP-2, the antibody of Toll-like receptor 2 extracellular domain, on the expression
and DNA-binding activity of nuclear factor-κB (NF-κB) p65 protein in mice with ulcerative colitis (UC). Methods Sixty BALB/c
mice were randomized equally into normal control group, UC model group, TSP-2 treatment group, and rabbit IgG treatment
group. In the latter 3 groups, the mice were fed with 5% DSS (C6H7Na3O14S3) solution for 7 days to induced UC, followed then
by treatment with daily injections of TSP-2 or rabbit IgG as appropriate for 7 days. The disease activity index was recorded
during the treatment. The colitis tissues were collected after the treatments for HE staining and detecting the expression and
DNA-binding activity of NF-κB p65 in the colon mucosa by Western blotting and ELISA. Results The DNA binding activity
and expressions of NF-κB P65 protein increased significantly in UC model group (P<0.05). TSP-2 treatment group significantly
decreased the disease activity index (P<0.05) and lowered the DNA-binding activity and expression of NF-κB P65 protein (P<
0.05) in the UC mouse models, while rabbit IgG produced no such effects (P>0.05). Conclusion TSP-2 can suppress the
DNA-binding activity and protein expressions of NF-κB P65 and regulate excessive immune response in the intestines to
ameliorate ulcerative colitis in mice.