Journal of Southern Medical University ›› 2013, Vol. 33 ›› Issue (03): 322-.
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Abstract: Objective To investigate the effect of maslinic acid preconditioning against injuries of rat cortical neurons inducedby oxygen-glucose deprivation (OGD). Methods The cortical neurons were isolated from Sprague-Dawley rat embryos at15-17 days of gestation for primary culture. The cortical neurons were incubated with different concentrations (0.1, 1, and 10μmol/L) of maslinic acid prior to OGD. The cell damage and viability were evaluated for lactate dehydrogenase (LDH) leakageand using MTT assay, respectively, and the expression of Bax protein was detected using Western blotting. Results OGDsignificantly increased LDH release rate and decreased the viability of the cells. After preconditioning with maslinic acid (1and 10 μmol/L), LDH leakage rate was decreased and cell the viability increased in cells exposed to OGD. Western blottingshowed that Bax expression in the cells decreased as maslinic acid concentrations increased. Conclusion Pretreatment withmaslinic acid can protect cultured embryonic rat cortical neurons against OGD-induced injury possibly in relation to decreasedexpression of Bax.
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https://www.j-smu.com/EN/Y2013/V33/I03/322