Journal of Southern Medical University ›› 2013, Vol. 33 ›› Issue (03): 322-.

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Protective effect of maslinic acid preconditioning against oxygen-glucose deprivationinduced injuries in embryonic rat cortical neurons

  

  • Online:2013-03-20 Published:2013-03-20

Abstract: Objective To investigate the effect of maslinic acid preconditioning against injuries of rat cortical neurons induced
by oxygen-glucose deprivation (OGD). Methods The cortical neurons were isolated from Sprague-Dawley rat embryos at
15-17 days of gestation for primary culture. The cortical neurons were incubated with different concentrations (0.1, 1, and 10
μmol/L) of maslinic acid prior to OGD. The cell damage and viability were evaluated for lactate dehydrogenase (LDH) leakage
and using MTT assay, respectively, and the expression of Bax protein was detected using Western blotting. Results OGD
significantly increased LDH release rate and decreased the viability of the cells. After preconditioning with maslinic acid (1
and 10 μmol/L), LDH leakage rate was decreased and cell the viability increased in cells exposed to OGD. Western blotting
showed that Bax expression in the cells decreased as maslinic acid concentrations increased. Conclusion Pretreatment with
maslinic acid can protect cultured embryonic rat cortical neurons against OGD-induced injury possibly in relation to decreased
expression of Bax.