Abstract: ObjectiveTo explore the feasibility of establishing an isolated porcine liver machine perfusion model and assess its
value in high-intensity focused ultrasound studies. MethodsTwenty-one isolated porcine livers were perfused with
autologous blood for4h through dual vessels (portal vein and hepatic artery) cannulation using an extracorporeal circulation
machine under a subnormothermic perfusion condition. The perfusion model was assessed by monitoring the liver color,
texture, liver weight gain, hemodynamic parameters, color Doppler flow imaging, bile output and histopathology. Results
Nineteen isolated porcine livers were successfully cannulated with dual vessels, and failure of hepatic artery intubation
occurred in two porcine livers. After machine perfusion for4h, the isolated livers maintained a soft texture with stable
hemodynamic levels within relative normal physiological ranges. The bile output was more than3ml/h within the initial3h of
perfusion. Histopathological examination demonstrated no morphological or structural changes of the liver tissues.
ConclusionThe isolated porcine liver perfusion model is stable and feasible, and can be used for high-intensity focused
ultrasound studies.