Abstract: ObjectiveTo investigate the effect of docetaxel on the interaction between C-jun and androgen receptor (AR) in
prostate cancer LNCaP cells and its androgen-independence subtype LNCaP-bic cells.MethodsLNCaP and LNCaP-bic cells
were treated with docetaxel and the changes in AR and AP-1expression were evaluated using luciferase assay. Western
blotting and immunoprecipitation assay were employed to analyze the effects of docetaxel on the expressions of C-jun and AR
and their interaction.ResultsLuciferase assay showed that LNCaP and LNCaP-bic cells expressed higher levels of AR and
C-jun after docetaxel treatment. Docetaxel induced a higher level of p-c-jun expression in LNCaP-bic cells than in the parental
LNCaP cells. Western blotting showed a strong PSA protein expression in LNCaP-bic cell after docetaxel treatment. Docetaxel
caused a stronger interaction between AR and C-jun in LNCaP-bic cells.ConclusionDocetaxel activates ligand-independent
AR transcription, and the interaction between AR and C-jun may affect the outcome of docetaxel chemotherapy.