Effects of recombinant human interleukin-10on tumor necorsis factor-α-induced adventitial fibroblast proliferationIn vitro

Abstract

Abstract: Objective To observe the effects of recombin an t human interleukin-10 (rhIL-10) on adventitial fibroblast prolifer-ation induce d by tumor necrosis factor-α(TNF-α)in vitro.Methods In this controlled stud y, NIH/3T3 cells cultured In vitrowere treated with TNF-αand recombinant human interleukin-10 (rhIL-10), respectively, and the cell proliferation was deter-mi ned by non-radioactive MTS/PES assay. Cell cycle analysis was performed using fl ow cytomertry.Results Compared with the control cells, TNF-αsignificantly sti mulated NIH/3T3 cell proliferation, wherea. rhIL-10 had no such effect. With TNF -αstimulation, rhIL-10 at the dose as low as 1 ng/ml inhibited the proliferatio n of NIH/3T3 cells (P<0.05). The number of cells in G₀/G₁ phase treated with both TNF-αand rhIL-10 was higher than those treated wit h TNF-αalone (P<0.05), as shown by flow cytometry.Conclusion rhIL-10 can sign ificantly inhibit the proliferation of adventitial fibroblasts induced by TNF-αIn vitro.

CLC Number:

R541

OUYANG Ping¹, MENG Su-rong¹, LIU Yan-bo¹, XU Ding-li¹, LAI Wen-yan¹, PENG Li-sheng², XU An-long². Effects of recombinant human interleukin-10on tumor necorsis factor-α-induced adventitial fibroblast proliferationIn vitro[J]. Journal of Southern Medical University, 2004, 24(01): 50-52.

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