Comparative studies of serological typing and HLA-A, B antigen genotyping with PCR using sequence-specific primers

Abstract

Abstract: Objective To evaluate the accuracy of PCR with sequence-specific primers (PCR-SSP) for HLA-Ⅰ genotyping and analyze the causes of the errors occurring in the genotyping.Methods DNA samples and were obtained from 34 clinical patients, and serological typing with monoclonal antibody (mAb) and HLA-A and, B antigen genotyping with PCR-SSP were performed.Results HLA-A and, B alleles were successfully typed in 34 clinical samples by mAb and PCR-SSP. No false positive or false negative results were found, and the erroneous and missed diagnosis rates were obviously higher in serological detection, being 23.5% for HLA-A and 26.5% for HLA-B. Error or confusion was more likely to occur in the antigens of A2 and A68, A32 and A33, B5, B60 and B61.Conclusion s DNA typing for HLA-Ⅰclass (A, B antigens) by PCR-SSP has high resolution, high specificity, and good reproducibility, which is more suitable for clinical application than serological typing. PCR-SSP may accurately detect the alleles that are easily missed or mistaken in serological typing.

CLC Number:

R446.11

WU Da-lin¹, LING Han-xin², TANG Hao¹. Comparative studies of serological typing and HLA-A, B antigen genotyping with PCR using sequence-specific primers[J]. Journal of Southern Medical University, 2004, 24(11): 1267-1270.

References

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