Journal of Southern Medical University ›› 2004, Vol. 24 ›› Issue (11): 1233-1235.

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Establishment and evaluation of the method for removing albumin and IgG in serum proteomics

LI Xue-hua1, LI Xin2, XU Shou-jun3, DING Yan-qing1   

  1. 1. 南方医科大学, 南方医院病理科, 广东, 广州, 510515;
    2. 南方医科大学, 肿瘤研究所, 广东, 广州, 510515;
    3. 军事医学科学院, 北京, 100850
  • Online:2004-11-20 Published:2004-11-20

Abstract: Objective To establish and evaluate the method for removing albumin and IgG in serum proteomics.Methods Elimination of serum albumin and IgG was achieved by filtering with Micro Bio-Spin column and the efficiency of purification evaluated by two-dimensional polyacrylamide gel electrophoresis (2-DE).Results The method for removing serum albumin and IgG was established, which enabled visualization of low-abundance proteins better in 2-DE map.Conclusion The established method for purifying serum proteins can be effectively applied in serum proteomic study of diseases.

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