Establishment of transgenic mouse model carring the promoter of plunc sequence

Abstract

Abstract: Objective To construct the vector using specific nasopharynx tissue promoter plunc to establish transgeneic mouse model. Methods Plunc-EGFP plasmid was digested with XhoⅠ, the purified linearized DNA fragments were recovered by gel extraction and diluted to the final concentration of 4 μg/ml, before introduced into fertilized one-cell mouse eggs by pronuclear microinjection. Results Thirteen founder mice were obtained, 12 of which were positive for the integrated EGFP gene as detected by PCR, and 3 were positive shown by Southern blotting. Conclusion Specific nasopharynx tissue promoter plunc can effectively induce exogenous gene integration into the mouse genome.

CLC Number:

R392-33

YANG Yu-fang, DING Yan-qing, ZHANG Ling, LIANG Li. Establishment of transgenic mouse model carring the promoter of plunc sequence[J]. Journal of Southern Medical University, 2004, 24(02): 155-157.

References

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