Cloning and sequence analysis of the genome of a strain of duck hepatitis B virus

Abstract

Abstract: Objective To clone and analyze the genome of a duck hepatitis B virus (DHBV) strain isolated from the ducks found in the local area. Methods The complete genome of DHBV was amplified by PCR prior to cloning into T vector and sequencing, with also homology and phylogenetic analyses. Result Genome comparison of an isolated DHBV strain and 16 DHBV strains in GenBank revealed a homology of 89.4% to 99.3% at the nucleotide level, and the amino acid identity of the 3 open reading frames showed that the P region harbored more obvious variations than the other regions. Conclusion The isolated DHBV strain might belong to a subtype of the virus found in the western countries.

CLC Number:

R512.6
Q78

LIANG Wei-fang, LIU Zhi-hua, HE Hai-tang, LUO Kang-xian. Cloning and sequence analysis of the genome of a strain of duck hepatitis B virus[J]. Journal of Southern Medical University, 2004, 24(04): 404-407.

References

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