Effects of exogenous tumor necrosis factor alpha on the invasiveness of human trophoblasts

Abstract

Abstract: Objective To observe the effect of exogenous tumor necrosis factor alpha (TNF-α) and its polyclonal antibody (anti-TNF-α) on the invasiveness of human trophoblasts in vitro. Methods The effect of exogenous TNF-αand anti-TNF-αon the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinases-2 (TIMP-2) in human trophoblastic cells was investigated with reverse transcription (RT)-PCR (RT-PCR). Results The trophoblasts cultured in vitro expressed MMP-2 but not TIMP-2. After incubation of the trophoblasts with 5 ng/ml TNF-αand TNF-αplus anti-TNF-αrespectively for 48 h, significant elevation of MMP-2 expression was induced, while the reverse occurred following incubation of the cells with 50 ng/ml anti-TNF-α. None of the treatments induced TIMP-2 expression. Conclusion Exogenous TNF-αmay promote the invasiveness of the trophoblasts, which can be inhibited by anti-TNF-α.

CLC Number:

CHEN Hui, PANG Zhan-jun, CHEN Shi-ling, XING Fu-qi. Effects of exogenous tumor necrosis factor alpha on the invasiveness of human trophoblasts[J]. Journal of Southern Medical University, 2004, 24(04): 397-399,407.

References

[1] Genhacev O, Di Federico E, Mc Master M, et al. Invasive cytotrophoblastapoptosisinpre-eclampsia[J]. HumReprod, 1999, 14(Supp12): 59-66.
[2] 程宝鸾.动物细胞培养技术[M].广州:华南理工大学出版社,2001.56-73.
[3] 孔令红,刘忠,陈士岭,等.人绒毛膜滋养层细胞的培养[J].第一军医大学学报,2000,20(1):10-1.Kong LH, Liu Z, Chen SL, et al. Culture of human trophoblastic cells[J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 2000,20(1): 10-1.
[4] 卢圣栋.现代分子生物学试验技术[M].第2版,北京:中国协和医科大学出版社,1999.458-65.
[5] BischofP, Meisser A, Campana A. Control of MMP-9 expression at the maternal-fetal interface[J]. J Reprod Immunol, 2002, 55 (1-2):3-10.
[6] BischofP, Campana Aldo. A putative role for oncogenes in tro-phoblast invasion[J]? Hum Reprod, 2000, 15 (Suppl 6): 51-8.
[7] Shimonovitz S, Hurwitz A, Hochnor D, et al. Expression of gelatinase B by trophoblast cells: down-regulation by progesterone[J].Am J Obstet Gynecol, 1998, 178(3): 457-61.
[8] Lukashev ME, Werb Z. ECM signalling: orchestrating cell behaviour and misbehaviout[J].Trends Cell Biol, 1998, 8(11): 437-41.
[9] Schwartz GK, Wang H, Lampen N, et al. Defining the invasive phenotype of proximal gastric cancer cells[J]. Cancer, 1994, 73(1):22-7.
[10] Fang J, Shing Y, Wiederschain D, et al. Matrix metalloproteinase-2is required for the switch to the angiogenic phenotype in a tumor model[J]. Proc Natl Acad Sci USA, 2000, 97(8): 3884-9.
[11] Meisser A, Chardonnens D, Campana A, et al. Effects of tumnout necrosis factor-alpha, interleukin-1 alpha, macrophage colony stimulating factor and transforming growth factor beta on trophoblastic matrix metalloproteinases[J]. Mol Hum Reprod, 1999, 5(3):252-60.
[12] Todt JC, Yang Y, Lei J, et al. Effects of tumor necrosis factor-alpha on human trophoblast cell adhesion and motility[J]. Am J Reprod Immunol, 1996 Aug, 36(2): 65-71.
[13] Yang Y, Yelavarthi KK, Chen HL, et al. Molecular, biochemical,and functional characteristics of tumor necrosis factor-alpha produced by human placental cytotrophoblastiic cells[J]. J Imanunol, 1993, 150(12): 5614-24.
[14] Knackstedt M, Ding JW, Arck PC, et al. Activation of the novel prothrombinase, fg12, as a basis for the pregnancy complications spontaneous abortion and pre-eclampsia[J]. Am J Reprod Immunol,2001,46(3): 196-210.