Ultrasound microbubbles promote thrombolysis of rabbit femoral artery thrombus

Abstract

Abstract: Objective To evaluate the efficacy of intravenous ultrasound microbubbles for thrombolysis of arterial thrombus without using thrombolytic drugs. Methods Twelve rabbit models of acute bilateral femoral artery thrombosis were established and 6 of them received transcutaneous ultrasound and intravenous albumin microbubble treatment for thrombosis on one side while only microbubble treatment for the other side. The other 6 rabbits received ultrasound treatment on one side but no treatment on the other to serve as the control group. Results None of the 6 arteries treated with microbubbles alone and only 2 arteries treated with ultrasound alone in the 6 control rabbits were recanalized. All the 6 femoral arteries treated with microbubbles together with ultrasound were recanalized (P=0.014), with significantly shorter patent time and smaller residual thrombus cross-sectional area than those of the arteries with only ultrasound treatment (P=0.004 and P=0.003, respectively). Conclusion Treatment with intravenous microbubbles assisted by transcutaneous ultrasound effectively promotes arterial thrombolysis in vivo, and this technique can be of significance in clinical treatment of acute thrombotic occlusions.

CLC Number:

OU Wen-chao, XIU Jian-cheng, XIE Jin-guo, ZHA Dao-gang, LIU Jian, HAO Rui, BIN Jian-ping, LIU Yi-li. Ultrasound microbubbles promote thrombolysis of rabbit femoral artery thrombus[J]. Journal of Southern Medical University, 2004, 24(05): 536-538.

References

[1] Suchkova V, Siddiqi FN, Carstensen EL, et al. Enhancement of fib rinolysis with 40-kHz ultrasound [J]. Circulation, 1998, 98 (10): 1030-5.
[2] Riggs PN, Francis CW, Bartos RS, et al. Ultrasound enhancement of rabbit femoral artery thrombolysis [J]. Cardiovasc Surg, 1997, 5(2): 201-7.
[3] Birnbaum Y, Luo H, Nagai T, et al. Noninvasive in vivo clot dissolution without a thrombolytic drug [J]. Circu lation, 1998, 97(2): 130-4.
[4] Porter TR, Xie F. Ultrasound, microbub bles, and thrombolysis [J]. Pro cardio vasc Dis, 2001, 44(1): 101-10.
[5] Nishioka T, Luo H, Fishbein MC, et al. Dissolution of thrombotic arterial oc clusion by high intensity low freqttency ultrasound and dodecafluoropentane emulsion: in vitro and in vivo study[J]. J Am Coll Cardiol, 1997, 30(2): 561-8.
[6] Sakuma T, Leong-Poi H, Fisher NG, et al. Further insights into the no-reflow phenomenon after primary angioplasty in acute myocardial infarc tion: the role of microthromboemboli [J]. J Am Soc Echocardiogr, 2003, 16(1): 15-21.
[7] 查道刚,刘伊丽,陈树元,等.超声照射对声振微泡稳定性的影响 [J].第一军医大学学报,1999,19(5):405-7 Zha DG, Liu YL, Chen SY, et al. Effect of ultrasound exposure on stability of sonicated microbubbles [J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 1999, 19(5): 405-7.
[8] 陈小林,贾满盈,刘伊丽,等静脉心肌声学造影评估冠脉血流储备的实验研究[J].第一军医大学学报,1997,17(4):290-3 Chen XL, Jia MY, Liu YL, et al. Myocardial contrast echocardiog raphy evaluation on coronary vessel blood flow reservation [J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 1997, 17(4): 290-3.
[9] Francis CW, Suchkova VN. Ultrasound and thrombolysis [J]. Vasc Med, 2001, 6(3): 181-7.
[10] Porter T, Kricsfeld D, Li S, et al. Comparison of d-dimer activity and residual particle size produced following thrombus disruption by perfluorocarbon containing microbubbles versus urokinase in thepresence of low frequency ultrasound [J]. J Am Coll Cardiol, 1998,31(2): 219.
[11] Suchkova V, Carstensen EL, Francis CW. Ultrasound enhancement of fibrinolysis at frequencies of 27 to 100 kHz Ultrasound Med Biol[J]. 2002, 28(3): 377-82.
[12] 杨远滨,周万松.低频超声的研究与应用进展[J]现代康复,2001,5(10):110-1.Yang YB, Zhou WS. The study and application of low frequency ul trasound[J]. Modem Rehabilit, 2001, 5(10): 110-1.
[13] 范鹰,关振中,于玲范,等体外治疗性超声助溶兔股动脉血栓[J].中国超声医学杂志,2000,16(5):335-7Fan Y, Guan ZZ, Yu LF, et al. Ultrasound promotes urokinase external therapeutic thrombolytic effect on rabbit femoral artery thrombosis [J]. Chin J Ultra Med, 2000, 16(5): 335-7.