Differentiation of bone marrow-derived stem cells in injured rat brain tissue

Abstract

Abstract: Objective To investigate the differentiation of bone marrow-derived stem cells (BMDSCs) migrated from blood circulation and resided in the injured brain tissue.Methods Brain injury model was established by iridectomy in the right cerebral cortex of female SD rats. Twenty-four hours after brain injury,the female rats received the implantation of green fluorescence protein (GFP)-labeled BMDSCs from male SD rats and were sacrificed at 2,4 and 8 weeks after the implantation. Fluorescent immunohistochemistry for CD11b and glial fibrillary acidic protein (GFAP) on the brain sections was used to detect the GFP-positive cells. Results One week after the transplantation of the GFP-labeled BMDSCs,3.53% of the peripheral blood white cells were GFP-positive; at 4 weeks and 8 weeks,a significant number of GFP-positive cells were found at the injury sites,some of which expressed CD11b and others expressed GFAP.Conclusion GFP-labeled BMDSCs can migrate to the injured brain tissue and differentiate into cells that express microglia- and astrocytes-specific antigens.

CLC Number:

Q254
Q189

ZOU Xi-feng¹, ZHANG Hai-yan², ZHAO Chun-li², LI Tie-lin¹, XU Qun-yuan². Differentiation of bone marrow-derived stem cells in injured rat brain tissue[J]. Journal of Southern Medical University, 2004, 24(10): 1102-1106.

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