Abstract: Objective To evaluate the value of color Doppler flow imaging (CDFI) in the diagnosis of testicular torsion, epididymitis, testitis, varicocele and testicular trauma. Methods The morphological changes of the testis and internal echo were displayed with two-dimentional ultrasound, and the blood perfusion in the testis was observed with color Doppler. The diagnoses were confirmed by operation and pathological examination or clinical follow-up. Results CDFI demonstrated reduction or disappearance of blood supply in the testis in 3 cases of testicular torsion, and increased blood flow was shown in 8 cases of testitis, 26 cases of epididymitis and 30 cases of. Nine cases of testicular trauma showed some spot-like blood supply or blood supply reduction in the testis. Conclusion CDFI is an inexpensive, practicable, reliable, and noninvasive method for diagnosing scrotal disease with reproducible results.