Journal of Southern Medical University ›› 2005, Vol. 25 ›› Issue (03): 305-307.

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Expression of imprinted gene Grb10 in human oocytes and preimplantation embryos

SHEN Wen-jie, KONG Ling-hong, CHEN Shi-ling, LI Hong, XING Fu-qi   

  1. 南方医科大学南方医院生殖中心, 广东, 广州510515
  • Online:2005-03-20 Published:2005-03-20

Abstract: Objective To investigate the mRNA expression of imprinted gene Grb10, a candidate gene for Silver-Russell syndrome (SRS), in human oocytes and preimplantation embryos for understanding the role of Grb10 in embryogenesis and assisted reproductive technology (ART).Methods Nested reverse transcriptase (RT)-PCR was used to analyze Grb10 gene expression in human oocytes and preimplantation embryos.Results The transcripts of Grb10 were detected in human oocytes and preimplantation embryos at all stages except for the 2-cell stage.Conclusion Grb10 gene is expressed in preimplantation embryos with close association with embryogenesis.

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