Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay

Abstract

Abstract: Objective To develop a method for quantitative detection of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) N protein by timed-resolved fluoroimmunoassay (TRFIA). Methods Using a monoclonal antibody (mAb) against SARS-CoV N protein, screened by SARS-CoV N protein and matching experiment, a method for quantitative detection of SARS-CoV N protein by TRFIA was established on the basis of double sandwich enzyme-linked immunosorbent assay (ELISA) and evaluated against the ELISA kit. Result The measurement range of the assay was 0.02-150 ng/ml with a sensitivity of 0.02 ng/ml, the coefficient of variability within runs of 3.3%-6.2%, and coefficient of variability between days of 5.3%-9.6%. The results of detection were consistent between ELISA and TRFIA. Conclusion TRFIA is a new, sensitive and specific immunoassay for detecting SARS N protein with potential value in clinical applications.

CLC Number:

R392.33

WANG Lei¹, WU Ying-song¹, TANG Yong-ping², LI Ming¹. Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay[J]. Journal of Southern Medical University, 2005, 25(04): 429-431,434.

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