Construction of eukaryotic expression vector for HPC2 and its expression in HEK293 cells

Abstract

Abstract: Objective To construct the eukaryotic expression vector for HPC2 for expression in HEK293 cells. Methods HPC2 from pcDNA3/HPC2 were inserted into the flag-tagged vector pcDNA3-flag by subcloning method. The recombinant plasmid pcDNA3-flag/HPC2 was then transfected into HEK293 cells using a routine lipofectamine method. The cell lysate was used for Western blotting to examine the expression of the target protein. Results and Conclusion Double restriction enzyme digestion and DNA sequencing indicated successful construction of the eukaryotic expression vector for HPC2 and the fusion protein was highly expressed in HEK293 cells, which provides an important basis for functional study of HPC2.

CLC Number:

TAN Kang-lian^1,2, LI Zhi-jie¹, LIU Jing-hua¹, HUANG Hao¹, TANG Jing¹, DENG Peng¹. Construction of eukaryotic expression vector for HPC2 and its expression in HEK293 cells[J]. Journal of Southern Medical University, 2005, 25(12): 1482-1484,1497.

References

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