Effect of trypsin and Triton-X 100 for decellularization of porcine aortic heart valves

Abstract

Abstract: Objective To test two decellularization procedures for their potential of cell removal and mormal matrix preservation. Methods Porcine aortic valve leaflets were treated with either 0.05% trypsin or 0.25% Triton-X 100 respertively for 48 h for decellularization and with fresh untreated valve leaflets as control. Two tissue samples from each group were stained with hematoxylin and eosin and observed light-microscopically followed by scanning electron microscopy. Ten valve leaflets in each group were measured for shrinkage temperature, tensile strength/fracture toughnes and percentage elongation. Results Trypsin and Triton-X 100 all achieved complete decellularization but Triton-X 100 caused stronger structural alterations. No significant difference was identified between untreated and trypsin groups in shrinkage temperature, tensile strength/fracture toughnes and percentage elongation, but Triton-X 100 group showed significant difference from the other two groups. Conclusion Decellularization using trypsin is superior to Triton-X100 in efficiency and matrix preservation.

CLC Number:

R318.11

WANG Ke-xue, ZHANG Jing-fang, ZHAN Qiu-peng, JIAN Xu-hua. Effect of trypsin and Triton-X 100 for decellularization of porcine aortic heart valves[J]. Journal of Southern Medical University, 2005, 25(01): 22-25.

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