Journal of Southern Medical University

Journal of Southern Medical University ›› 2006, Vol. 26 ›› Issue (12): 1803-1805.

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Construction of a recombinant HBV DNA vaccine and its evaluation for specific cellular immune response in mice

GUO Yan1,WANG Ju-fang2,LUO Xiao-chun2,SU Jin3,MA Li3,ZHANG Yang-de1 1Xiangya Medical School of Central South University,Changsha 410078,China;2School of Biosciences and Bioengineering,South China University of Technology,Guangzhou 510640,China;3Institute of Molecular Immunology,Southern Medical University,Guangzhou 510515,China   

  1. 中南大学湘雅医学院生物医学工程系； 华南理工大学生物科学与工程学院； 南方医科大学分子免疫研究所； 中南大学湘雅医学院生物医学工程系 湖南长沙410078； 广东广州510640； 广东广州510515； 湖南长沙410078；
  • Online:2006-12-20 Published:2006-12-20

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Abstract: Objective To study the specific cellular immune response induced by a recombinant HBV DNA vaccine and evaluate the potential therapeutic effect of this vaccine against HBV.Methods A series of HBV epitopes were screened and the optimal combinations of these gene fragments identified to construct the target gene HS which incorporated appropriate flanking sequences.HS was inserted in pVAX1 to construct the recombinant plasmid PVAX-HS.Generation of specific cytotoxic T lymphocytes(CTLs)induced by PVAX-HS in HLA-A2 mice was observed by 51Cr assay and the activity of the CTLs evaluated using ELISPOT assay.Results PVAX-HS was constructed successfully,which induced specific CTLs with strong activities after immunization in mice as compared with the control group that yielded negative results.Conclusion PVAX-HS DNA immunization can induced specific cellular immune response in mice,suggesting the potential therapeutic effect of this HBV vaccine.

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