Abstract: Objective To study the durability of expanded polytetrafluoroethylene artificial heart valve(ePTFE AHV).Methods Six ePTFE AHVs were tested for 400 million times against accelerated fatigue using TH-2200 artificial heart valve exosomatic accelerated fatigue instrument.Hydromechanical parameters of fore-and-aft accelerated fatigue test of the 6 AHVs were obtained by TH-1200 artificial heart valve exosomatic pulsatile stream instrument.Results and Conclusion The mean gradient pressure spanning the valve and the effective orific area of ePTFE AHVs did not undergo significant changes after fore-and-aft the fatigue test,but the regurgitation volume and regurgitation rate of ePTFE AHVs were reduced after the accelerated fatigue test,suggesting good durability of ePTFE AHV.