In vitro study of cytotoxic T lymphocyte activation by antigen-loaded dendritic cells for killing of K562 cells

Abstract

Abstract: Objective To study the effect of dendritic cells(DC)stimulated with K562 cell lysate in inducing specific cytotoxic T lymphocytes(CTL)against K562 cells in vitro.Methods The DCs were derived from healthy human peripheral blood monocytes in the presence of granulocyte-macrophage colony-stimulating factor,interleukin(IL)-4 and tumor necrosis factor(TNF)α.The T cells were stimulated by DCs loaded with freeze-thawed K562 cells and T-cell cytotoxicities were measured by lactate dehydrogenase(LDH)assay.Results The DCs could be successfully obtained from peripheral blood monocyte after the culture.Mixed lymphocyte reactions induced by the antigen-loaded DC were much stronger than those induced by human peripheral blood monocytes(P<0.05).At the effector to target ratio of 10:1 and 20∶1,cytotoxicities against K562 cells by CTL derived from culture with the antigen-loaded DCs were the strongest(P<0.05).Conclusion CTL derived from DCs pulsed with K562 cell lysate show effecitive and specific cytotoxicity against K562 cells.

CLC Number:

R392

HUA Jia-ye1,HUANG Ren-wei2 1Second Affiliated Hospital of Guangzhou Medical College,Guangzhou 510260,China;2Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China. In vitro study of cytotoxic T lymphocyte activation by antigen-loaded dendritic cells for killing of K562 cells[J]. Journal of Southern Medical University, 2006, 26(05): 617-619.

References

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