Journal of Southern Medical University

Journal of Southern Medical University ›› 2022, Vol. 42 ›› Issue (3): 418-424.doi: 10.12122/j.issn.1673-4254.2022.03.15

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Whole-transcriptome sequencing analysis of placental differential miRNA expression profile in Down syndrome

HE Jianping, TANG Jian, SU Hong, SHEN Cuihua, LUO Shengjun, WANG Haitao, QIAN Yuan, LÜ Mengxin   

  1. Department of Medical Genetics and Prenatal Diagnosis, Genetic Counseling Clinic, Department of Obstetrics, Department of Pathology, Kunming Maternal and Child Health Care Hospital, Kunming 650031, China
  • Online:2022-03-20 Published:2022-04-11

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Abstract: Objective To identify new biomarkers and molecular pathogenesis of Down syndrome (DS) by analyzing differentially expressed miRNAs in the placentas and their biological pathways. Methods Whole transcriptome sequencing was used to identify the differentially expressed miRNAs in DS (n=3) and normal placental samples (n=3) diagnosed by prenatal diagnosis. The target genes were predicted using miRWalk, Targetscan and miRDB, and GO and KEGG pathway analyses were performed for gene enrichment studies. Results We identified a total of 82 differentially expressed miRNAs in the placental tissues of DS, including 29 up-regulated miRNAs (fold change ≥2, P<0.05) and 15 down-regulated miRNAs (fold change ≥2, P<0.05), among which 10 miRNAs with relatively high expression abundance were selected for further analysis, including 4 up-regulated and 6 down-regulated miRNAs. These selected miRNAs shared the common target genes BTBD3 and AUTS2, both of which were associated with neurodevelopment. GO analysis showed that the target genes of the selected miRNAs were mainly enriched in protein binding, hydrolytic enzymes, metal ion binding protein combining, transferase activity, nucleotide, cytoplasmic constituents, nucleus composition, transcriptional regulation, RNA metabolism regulation, DNA-dependent RNA polymerase II promoter transcriptional regulation, eye development, and sensory organ development. KEGG enrichment analysis showed that the target genes of these differentially expressed miRNAs were involved in the signaling pathways including tumor-related signaling pathway, PI3K-Akt signaling pathway, Ras signaling pathway, Rap1 signaling pathway, cytoskeletal regulatory signaling pathway, purine metabolization-related signaling pathway and P53 signaling pathway. Conclusion The differentially expressed miRNAs may play important roles in placental damage and pregnancy pathology in DS and provide clues for the prevention and treatment of mental retardation-related diseases.

Key words: Down syndrome; microRNA; transcriptome sequencing; placenta