Abstract: Objective To investigate the prevalence of physical state of HPV-l6 DNA in cervical cancer and cervical precancerous
carcinoma. Methods Multiplex PCR was adopted to detect the physical state of HPV in samples from 252 patients with
cervical carcinoma, including 48 samples of cervical cancer, 204 cervical intraepithelial neoplasia (CIN ) (125 CIN I, 46 CIN II
and 33 CIN III) and 20 normal samples from the subjects with hysteromyoma undergoing hysterectomy, respectively. Results
Among 48 patients with cervical cancer, 31 (65.6%) were infected with HPV-16. Eighteen among 31 (58.1%) HPV-16 infected
patients with cervical cancer were found to have integrated infection of HPV-16. The positive rates of HPV-16 infection in the
patients with CIN I, CIN II and CIN III were 19.2%, 34.8% and 42.4%, and the integrated infection rates of HPV-16 were 16.7%,
18.8% and 35.7%, respectively. Compared with patients with different grades of CIN, the integrated rate of HPV-16 infection in
those with cervical cancer was significantly elevated. Conclusion Among the patients with HPV-16 infection, the integrated
state of HPV-16 is positively correlated with the severity of cervical lesions. Combined HPV typing test and detection of
integrated viral state contribute to predicting the prognosis of patients with cervical precancerous lesions and increasing the
accuracy of screening cervical cancer on the basis of HPV DNAdetection.