Journal of Southern Medical University ›› 2014, Vol. 34 ›› Issue (11): 1589-.
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Abstract: Objective To examine the changes in the expression of pro-inflammatory and anti-inflammatory cytokines in theperipheral blood mononuclear cells (PBMCs) and elucidate the inflammatory status of apparently healthy subjects. MethodsPeripheral blood samples (5 ml) were collected after fasting for more than 8 h from 14 healthy control subjects and 14apparently healthy subjects with elevated serum high-sensitivity CRP (hsCRP) level (≥2.0 mg/L). PBMCs were separated byFicoll density gradient centrifugation and the total RNA was extracted for real-time quantitative PCR analysis of theinflammatory cytokines, and plasma was separated for ELISA analysis of interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) expressions. Results The gene expressions of TNF-α and MCPIP were significantly increased in PBMCs of apparentlyhealthy subjects, while IL-6 and MCP-1 only showed slight elevations; IL-10 expression in PBMCs decreased significantly inapparently healthy subjects as compared to that in the control group. The results of ELISA showed significantly elevatedTNF-α level without significant changes of plasma IL-6 level in apparently healthy subjects. Conclusion In apparently healthysubjects with normal lipid levels, chronic low-grade inflammation has occurred shown by elevated expressions of thepro-inflammatory cytokines and lowered expressions of the anti-inflammatory cytokines.
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https://www.j-smu.com/EN/Y2014/V34/I11/1589