Abstract: ObjectiveTo assess the therapeutic effect of intramuscular transplantation of bone-marrow derived mesenchymal
stem cells (BM-MSCs) in the leg for treatment of diabetic foot ulcers (DFUs) in rats.MethodsThirty-six male Wistar rats were
randomly allocated into 3 equal groups, namely group A with DFUs on the bilateral hindlimb dorsum pedis and
intramuscular transplantation of 4,6-diamino-2-phenylindole (DAPI)-labeled third-passage BM-MSCs from male Wistar rats
into the leg, group B with nondiabetic foot ulcers, and group C with DFUs but without BM-MSC transplantation. On days2, 5,
8and11posttransplantation, the rate of wound healing was evaluated, the labeled BM-MSCs in the wound tissues were traced
on frozen sections, and the thickness of granulation tissues was measured with HE staining. Immunohistochemistry was
performed to detect the expression of CD31and Ki-67, and the expression of vascular endothelial growth factor (VEGF) in the
wound tissues was detected by ELISA and RT-PCR.ResultsThe rats in group B showed significantly faster wound closure
than those in the other two groups (P<0.05). The rate of wound healing was greater in group A than in group C on days8and
11(P<0.05). The fluorescence intensity and area on the frozen sections were the highest on day5posttransplantation in group
A, which had a granulation tissue thickness comparable with group B but greater than group C. The mean numbers of small
blood vessels and cells positive for CD31 and Ki-67 expressions were similar between groups A and B, which showed
significant differences from group C (P<0.05). On day11, group A showed the highest VEGF expression in the wound tissues
(P<0.001). ConclusionIntramuscular transplantation of BM-MSCs can significantly promote wound healing of DFUs in rats
possibly as a result of increased expression of VEGF in the wound tissues.