Abstract: Objectiveo investigate the distribution and drug sensitivity of pathogenic bacteria isolated from patients with
nosocomial infection in intensive care unit.MethodsThe clinical data were collected from229hospitalized patients with
nosocomial infection in intensive care unit from2009to 2011. ResultsGram-negative bacteria were the main pathogens
(68.25% ) in the intensive care unit. From2009to 2011, the annual isolation rate of multidrug-resistant baumanii showed a
significant reduction with time (39.13% ,18.18% , and15% , respectively,P<0.05), while the prevalence of multidrug-resistant
Pseudomonas aeruginosa, ESBLs-producing Escherichia coliandKlebsiella pneumoniae, methicillin-resistant Staphylococcus aureus,
coagulase-negative staphylococci methicillin-resistant strains showed no significant changes in the3years (P>0.05). The overall
sensitivity of gram-negative bacilli to the antimicrobial agents tended to increase with time. The Gram-negative bacilli
remained highly sensitive to carbapenems, tigecycline, amikacin and piperacillin/tazobactam; the Gram-positive bacilli were
highly sensitive to vancomycin, quinoline Nupu Ting/dalfopristin, linezolid, and tigecycline with sensitivity rates all reaching
100% .ConclusionAdherence to the principles of antibiotic use and effective monitoring and preventive measures are
encouraged to reduce antibiotic resistance rates of the bacteria and the incidences of hospital infection.