Abstract: Objective To standardize the establishment of a stable rat model of orthotopic liver transplantation and surgical technique of two-cuff technique for improving the operation success rate,and compare the effect of graft perfusion via the portal vein and the abdominal aorta on the graft function.Methods Totally 100 cases of orthotopic liver transplantation was performed in rats under microscope according to modified Kamada’s two cuff technique.The rats were divided into 2 groups with the donor liver perfused through the portal vein with 10 ml cold lactated Ringer’s solution,and via the abdominal aorta with 20 ml cold lactated Ringer’s solution,respectively.The postoperative function recovery and pathological changes of the liver grafts were evaluated by serum ALT detection and histopathological examination.The operation success rate,3-month survival rate of the rats and the complications were observed.Results No significant differences was noted in the liver function,operation success rate and 3-month survival rate between the 2 groups,and histopathological examination also showed similar findings.The success rates of the two groups were 98% and 96%,with 3-month survival rate of 93.5%(29/31) and 93.3%(28/30)(P>0.05),respectively.Conclusion The liver transplantation models with portal vein or abdominal aorta graft perfusion both serve well their respective purposes.Good microsurgical skills,standardized performance and shortened anhepatic period are the keys to improved stability and survival rate and reduced operative complications.