Abstract: Objective To improve the method for preparing rabbit VX2 liver tumor model and observe the magnetic resonance imaging (MRI) features of the implanted tumors. Methods Sixteen adult New Zealand white rabbits were assigned randomly into 4 equal groups, and VX2 tumor tissues were implanted into the right and left liver lobes with spiral CT guidance. Plain and contrast-enhanced MR scann and pathological analysis were performed in different stages (14, 18, 22 and 26 days) after tumor implantation. Results Tumor implantation was successful in all the rabbits, and 18 to 22 days after tumor implantation, the diameters of the tumor ranged from 1 to 2 cm, which allowed observation and study. In plain MR scans, lower or equivalent tumor signal in comparison with hepatic parenchyma was observed, and contrast-enhanced scans produced obvious enhancement of the tumor edges. At 22 days after tumor implantation, obvious necrosis was observed in the center of the tumor. Conclusion This method of preparing rabbit VX2 liver tumor model with spiral CT guidance is simple and convenient, and the tumors can be observed effectively with dynamic plain and contrast-enhanced MR scans.