Induced differentiation of human cord blood monocytes into neuron-like cells in vitro

Abstract

Abstract: Objective To probe the conditions for inducing human cord blood monocytes to differentiate into neuron-like cells. Methods The mononuclear cells were isolated from human umbilical cord blood samples and plated in 25-mm culture flasks containing DMEM/F12 medium. The fifth passage of mesenchymal stem cells(MSCs) were induced by β-mercaptoe-thanol(β-ME), dimethyl sulfoxide(DMSO) and conditioned medium for neuron induction, respectively, to differentiate into neuron-like cells. The expression of nestin, neuron-specific enolase(NSE) and neurofilament(NF) on the treated cells were detected by immunocytochemical method. Results Nestin expression were found in 6.7% of the primary, 12.4% of the second passage, 20.8% of the fifth passage of MSCs, respectively. Thirty-six percent of cell cultures treated with the conditioned medium for neuron induction were immunoreactive for NSE and NF, and 33% of the cells induced by β-ME and 25% of the cells by DMSO also expressed NSE and NF. Conclusion Cord blood MSCs possess some features of neural stem cells, and have the capacity to differentiate into neuron-like cells under proper conditions.

CLC Number:

R329.24

LIU Tai-yun¹, ZHANG Cheng¹, XIAO Lu-lu², YAO Xiao-li¹, FENG Shan-wei¹, ZENG Ying¹. Induced differentiation of human cord blood monocytes into neuron-like cells in vitro[J]. Journal of Southern Medical University, 2005, 25(02): 152-155.

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