Advanced oxidation protein products induce reactive oxygen species production in endothelial cells

Abstract

Abstract: Objective To investigate the effect of advanced oxidation protein products (AOPP) on production of reactive oxygen species (ROS) in endothelial cells. Methods Human umbilical vein endothelial cell line ECV304 were stimulated with AOPP-modified bovine serum albumin (AOPP-BSA) prepared by oxidation of BSA with HOCl in vitro. ROS production in the cells was evaluated by kinetic measurement of dichlorofluoroscein (DCF) fluorescence produced by oxidation of an oxidant-sensitive dye 2,7-dichlorefluorescin (DCFH) using VICTOR 1420 multilabel counter. Results AOPP-BSA could induce ROS production in ECV304 cells time- and dose-dependently, and the quantity of ROS increased with the oxidation degree of BSA. Pretreatment of cells with a small-molecular-mass glutathione peroxidase mimic ebselen or NADPH oxidase inhibitor apocynin inhibited ROS production by 65% and 29% respectively. Conclusion AOPP-BSA induces ROS production in endothelial cells, partially mediated by NADPH oxidase activation.

CLC Number:

Q251

YUAN Fang, LIU Shang-xi, TIAN Jian-wei. Advanced oxidation protein products induce reactive oxygen species production in endothelial cells[J]. Journal of Southern Medical University, 2004, 24(12): 1350-1352.

References

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