Construction of a three-dimensional human angiogenesis model in vitro for antiangiogenic drug selection

Abstract

Abstract: Objective To develop a simple human angiogenesis model that simulates all the critical steps of the angiogenic process for screening antiangiogenic substances.Methods Human umbilical vein endothelial cells (HUVECs) were sandwiched into three-dimensional collagen gels for culturing to produce the angiogenesis model in vitro,on which the antiangiogenic effects of genistein,tyrphostin A23 and lavendustin C were assessed. Results HUVECs had the capacity to repidly form highly organized capillary-like structures in this model with uniformity of the entire culture,and was easily reproducible. Tyrosine kinase inhibitors,genistein,tyrphostin A23 and lavendustin C were found to inhibit the capillary tube formation.Conclusion This new human angiogenesis model has proved to be simple,responsive,and reproducible for testing potential angiogenic inhibitors,and is suitable for screening anti-angiogenic drugs.

CLC Number:

R733.7

LI Bin. Construction of a three-dimensional human angiogenesis model in vitro for antiangiogenic drug selection[J]. Journal of Southern Medical University, 2004, 24(10): 1167-1170.

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