Exogenous mTκα gene inhibits the growth of HepG2 heptocellular carcinoma cells

Abstract

Abstract: Objective To observe the expression of mTκα,the inhibitor of NFκB,in HepG2 heptocellular carcinoma cells and the inhibitory effect of mTκα;α on HepG2 cell growth.Methods The adenovirus containing mTκα or the packaging vector were harvested from 293 packaging cells to infect HepG2 cells,in which the expression of green fluorescence protein (GFP) and mIκBα protein were detected. The plating efficiency and colony-forming ability in soft agar were evaluated,cell growth curve was generated and the tumor growth observed in nude mice. Results HepG2 cells infected by the packaged adenovirus (HepG2/Adv cells) had positive GFP expression,and those infected by the adenovirus containing mIκBα (HepG2/Ad-mIκBα cells) showed mIκBα protein expression,as demonstrated by Western blot analysis. HepG2/Adv cells exhibited markedly lowered colony-forming ability as compared with that of the HepG2/Adv and HepG2 cells,and the latter two cells,but not the former cells,could survive in soft agar and form colonies in the shape mulberries. Cell growth curve showed that the Hep G2/Ad-mIκBα cells had also significantly reduced growth rate,but their saturation density in culture was comparable with the other two cells. Four weeks after inoculation in nude mice,HepG2/Ad-mIκBα and HepG2/Adv cells showed no significant difference in tumorigenicity (80%vs100%,respectively),but the tumor volume generated by the former cells was significantly smaller (P<0.05).Conclusion mTκα gene can inhibit the growth of HepG2 heptocellular carcinoma cells.

CLC Number:

R392.
R735.7

JIN Wu-dong¹, CHEN Long-hua¹, MU Feng². Exogenous mTκα gene inhibits the growth of HepG2 heptocellular carcinoma cells[J]. Journal of Southern Medical University, 2004, 24(10): 1133-1136.

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