Journal of Southern Medical University

Journal of Southern Medical University ›› 2021, Vol. 41 ›› Issue (8): 1277-1282.doi: 10.12122/j.issn.1673-4254.2021.08.22

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Surface modification of titanium implant with hBMP-2/hIGF-1 for promoting biocompatibility and osteogenesis

ZHANG Di, DENG Tian, LUO Zhen, ZHU Andi, YANG Bo, ZHONG Huilan, LI Shaoping, YANG Xiaoyu   

  1. Department of Implantology, Stomatological Hospital, Southern Medical University, Guangzhou 510280,China; Department of Stomatology, Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen 518107, China
  • Online:2021-08-20 Published:2021-09-07

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Abstract: Objective To prepare the human bone morphogenetic protein-2 (hBMP-2)/ human insulin-like growth factor-1 (hIGF-1) coating titanium (Ti) and assess its performance as a dental implant material. Methods hBMP-2 and hIGF-1 were coated to the smooth surface of a Ti plate, and its efficacy for promoting bone formation and bone integration was compared with a pristine Ti plate. The surface characteristics of the metal samples were evaluated using scanning electron microscope (SEM) and by contact angle measurement. MG63 cells were seeded on the surface of the Ti plates, and MTT assay and alizarin red staining was used to examine the cell proliferation and formation of calcified nodules, respectively. Alkaline phosphatase (ALP) secretion of the cells was examined with ELISA, and cellular expressions of osteocalcin and osteopontin were detected with Western blotting for assessing osteogenesis. Results SEM examination showed that the surface of Ti with hBMP-2 and hIGF-1 coating presented with a radial pattern resembling snowflakes. The contact angles of non-coated Ti, hBMP-2-coated Ti, hIGF-1-coated, and hBMP-2/-hIGF-1-coated Ti samples were 83.2°, 54°, 56° and 54°, respectively. Compared with the non-coated Ti plate, the surface-modified Ti samples showed a significantly smaller contact angle (P=0.032, 0.029, and 0.028), indicating a good hydrophilicity of the samples. MTT assay showed that MG63 cells grew well on the surface of the coated Ti plates. The hBMP-2/IGF-1 coating significantly induced cellular secretion of ALP (P=0.021, 0.014) and obviously promoted osteogenesis of MG63 cells (P<0.05). Western blotting results showed that hBMP-2/IGF-1 coating significantly enhanced the expressions of osteocalcin and osteopontin in the seeded cells (P<0.05). Conclusion hBMP-2 and hIGF-1 coating of Ti material can promote osteogenesis of the cells seeded on its surface to improve the performance of such Ti material as dental implants.

Key words: human bone morphogenetic protein-2; insulin-like growth factor-1; heparin; osteogenesis; osseointegration