南方医科大学学报

南方医科大学学报 ›› 2020, Vol. 40 ›› Issue (01): 56-60.doi: 10.12122/j.issn.1673-4254.2020.01.08

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抑郁倾向Itpr2-/-小鼠海马3D-ASL灌注成像改变

曾善美，刘 恺，张竞予，吴玉兰，徐一华，孙学刚，文 戈   

  • 出版日期:2020-02-18 发布日期:2020-01-20
  • 基金资助:

Changes in three-dimensional arterial spin labeling perfusion imaging of the hippocampus in depressive Itpr2 -/- mice

  

  • Online:2020-02-18 Published:2020-01-20

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摘要： ：目的 研究肌醇1,4,5-三磷酸受体2型（Itpr2）全身敲除小鼠（Itpr2-/-小鼠）行为学变化，并利用三维动脉自旋标记（3D-ASL）成像探究海马血流灌注量可能存在的异常情况。方法 将28只Itpr2-/-小鼠作为实验组，20只野生型小鼠作为对照组，采用行为学测试的方法对实验组Itpr2-/-小鼠及对照组野生型小鼠进行抑郁倾向的考察，测试内容包括糖水偏爱实验、悬尾实验、强迫游泳实验以及旷场实验。分别随机选取15只Itpr2-/-小鼠及14只野生型小鼠，进行3D-T2WI结构图像及海马中间层面的3D-ASL图像的采集，计算得出该海马层面的血流灌注图（CBF图），利用ITK-SNAP手动勾画左右海马区域并测出其左侧海马、右侧海马的血流灌注值（CBF值）。结果 行为学测试结果显示Itpr2-/-小鼠出现明显抑郁倾向，糖水偏爱测试显示Itpr2-/-小鼠糖水偏好百分比下降（P<0.05），悬尾实验中Itpr2-/-小鼠的不动时间延长（P<0.05），强迫游泳实验中中Itpr2-/-小鼠的不动时间延长（P<0.01），而旷场实验中两组小鼠行为没有明显差异（P>0.05）。相较于正常对照组小鼠，Itpr2-/-小鼠左侧（P<0.01）及右侧（P<0.01）海马区平均血流灌注量下降，其中实验组Itpr2-/-小鼠及正常对照组小鼠的左侧海马CBF平均值分别为73.30±5.61和95.77±5.10，右侧海马CBF平均值分别为73.53±5.70和100.5±4.70，双侧海马最终计算所得平均值分别为73.42±5.61和98.12±4.75。结论 Itpr2的缺失可能与小鼠抑郁倾向相关，且对海马的平均血流灌注量存在影响。

Abstract: Objective To study the behavioral changes of inositol 1,4,5-trisphosphate receptor type 2 knockout (Itpr2 -/- mice) and investigate the blood perfusion changes in the hippocampus using three-dimensional arterial spin labeling (3D-ASL). Methods 28 Itpr2 -/- mice and 20 wild-type mice were assessed for depressive phenotype using behavioral tests (including sucrose consumption test, tail suspension test, forced swimming test and open field test). 15 Itpr2 -/- mice and 14 wild- type mice were randomly selected for 3D-T2WI imaging of the whole brain and 3D-ASL imaging of the middle hippocampal layer, and cerebral blood flow (CBF) of the middle hippocampal layer was calculated. ITK-SNAP was used to delineate the bilateral hippocampal area and measure the average CBF value. Results Compared with the wild- type mice, Itpr2 -/- mice exhibited a distinct depressive phenotype with significantly decreased sucrose preference (P<0.05) and increased immobile time in tail suspension test (P<0.05) and forced swimming test (P<0.01), without obvious changes in the performance in open field test (P> 0.05). Significantly decreased mean CBF values were found in the left and right hippocampus of Itpr2 -/- mice as compared with the wild-type mice (left: 73.30 ±5.609 vs 95.77±5.095; right: 73.53±5.700 vs 100.5±4.696; bilateral means: 73.42±5.607 vs98.12±4.754; P<0.01). Conclusion Itpr2 deficiency can cause depressive phenotype and affect the cerebral blood flow in the hippocampus of mice.