南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (07): 1063-.

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SFRP4和DKK1在宫颈鳞癌中的表达及临床病理意义

李秀芬，侯安丽，李振环，方淑芹，张雅丽，徐亚威   

  • 出版日期:2015-07-20 发布日期:2015-07-20

Expression of SFRP4 and DKK1 in cervical squamous cell carcinoma and the
clinicopathological implications

  • Online:2015-07-20 Published:2015-07-20

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摘要： 目的研究分泌型卷曲相关蛋白（SFRP4)及Wnt信号通路抑制因子DKK1（Dickkopf-1）在宫颈鳞癌中的表达及临床病理
意义。方法采用逆转录聚合酶链式反应(RT-PCR)检测66例宫颈鳞癌组织中及26例正常宫颈组织中DKK1和SFRP4 mRNA
的表达水平；采用免疫组织化学法检测66例宫颈鳞癌组织中及26例正常宫颈组织中DKK1和SFRP4蛋白的表达水平。应用
SPSS17.0统计学软件进行统计学分析，研究DKK1和SFRP4的表达与宫颈鳞癌患者主要临床病理指标的关系。结果宫颈鳞
癌SFRP4表达高于正常宫颈（P<0.01）。宫颈鳞癌DKK1表达低于正常宫颈（P<0.05）。DKK1和SFRP4在宫颈鳞癌中的表达与
临床病理分期、组织分化程度、有无淋巴转移及浸润深度有关（P均<0.05）。DKK1与SFRP4在宫颈鳞癌中的表达呈负相关（P<
0.01）。结论Wnt信号通路上游因子SFRP4和DKK1在宫颈鳞癌发生中起关键作用，并且与宫颈鳞癌的临床病理指标密切相关。

Abstract: Objective To investigate the expression of SFRP4 and DKK1 in cervical squamous cell carcinoma and explore the
clinicopathological implications. Methods Immunohistochemistry and reverse transcriptase-polymerase chain reaction
(RT-PCR) were used to detect the expressions of SFRP4 and DKK1 in 66 cervical squamous cell carcinoma and 26 normal
cervical specimens. Results SFRP4 expression was significantly higher (P<0.01) and DKK1 expression was significantly lower
(P<0.05) in the carcinoma tissues than in normal cervical tissues. DKK1 was negatively correlated with SFRP4 in the carcinoma
tissues (P<0.01), and their expressions were associated with the clinical stages, tumor differentiation, depth of invasion and
lymph node metastasis of the tumors (P<0.05). Conclusion SFRP4 and DKK1, the upstream components of the Wnt pathway,
play a key role in the tumorigenesis of cervical squamous cell carcinoma, and their expressions are associated with the
clinicopathological features of the malignancy.