南方医科大学学报

南方医科大学学报 ›› 2023, Vol. 43 ›› Issue (11): 1850-1856.doi: 10.12122/j.issn.1673-4254.2023.11.03

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环状RNA hsa_circ_0006834可作为肝细胞癌患者预后的潜在生物标志物

张 铃，赵春雨，许瑶瑶，陈炎森，蔡志雄，林浩伟，蔡巧燕   

  1. 福建中医药大学中西医结合研究院，福建 福州 350122；福建省中西医结合老年性疾病重点实验室，福建 福州 350122；福建中医药大学中西医结合学院，福建 福州 350122；福建医科大学孟超肝胆医院，福建 福州 350000
  • 出版日期:2023-11-20 发布日期:2023-12-08

Circular RNA hsa_circ_0006834 is a potential prognostic biomarker for hepatocellular carcinoma

ZHANG Ling, ZHAO Chunyu, XU Yaoyao, CHEN Yansen, CAI Zhixiong, LIN Haowei, CAI Qiaoyan   

  1. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; Fujian Provincial Key Laboratory of Integrative Medicine on Geriatrics, Fuzhou 350122, China; College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; Meng Chao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350000, China
  • Online:2023-11-20 Published:2023-12-08

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摘要： 目的 基于RNA测序技术筛选肝细胞癌（HCC）中差异表达的环状RNAs（circRNAs），检测其在HCC组织中的表达变化，并探讨其与临床特征及预后的关系，为HCC提供潜在的分子标志物和治疗靶点。方法 选取56对HCC组织及其癌旁组织进行RNA测序，筛选出具有差异表达的 circRNAs；采用RT-qPCR法在另外选取的15对HCC组织和配对的癌旁组织中对circRNA进行差异表达的验证；分别运用卡方、Cox比例风险模型及Kaplan-Meier法分析circRNA与HCC患者临床特征及预后的相关性；采用ROC曲线评估circRNA对HCC患者无复发生存期和总体生存期的预测价值。结果 基于56对HCC组织及其癌旁组织的RNA测序数据分析显示：在HCC组织中显著上调的circRNAs有17个，显著下调的circRNAs有 45个（P<0.05）；其中circRNA hsa_circ_0006834在HCC中异常低表达，并在另外15对HCC组织及其癌旁组织中进一步验证其在癌组织中显著低表达（P<0.05）。结合临床数据分析显示hsa_circ_0006834的表达与HCC患者的血管侵犯和巴塞罗那（BCLC）分期呈显著负相关，与无复发生存期和总体生存期呈显著正相关（P<0.05），可作为独立的预后因素。ROC曲线预测2年无复发生存期和总体生存期的AUC面积分别为0.750和0.7601，具有良好的预后评估效能。结论 hsa_circ_0006834在HCC组织中低表达，具有作为HCC患者预后评估的新型生物标志物的潜力。

关键词: 肝细胞癌；环状RNA；hsa_circ_0006834；预后；标志物

Abstract: Objective To explore the differentially expressed circular RNAs (circRNAs) in hepatocellular carcinoma (HCC) and their association with clinical features and prognosis of HCC patients. Methods RNA sequencing was performed on 56 pairs of HCC and adjacent tissues to identify the differentially expressed circRNAs in HCC, whose expressions were validated by RT-qPCR in another 15 pairs of HCC and adjacent tissues. The correlation of the selected circRNAs with the clinical features and prognosis of the patients was analyzed using Chi-square test, Cox proportional hazards regression model and Kaplan-Meier analysis. The predictive value of the identified circRNA for recurrence-free survival and overall survival of HCC patients was assessed using ROC curves. Results We identified 17 significantly up-regulated and 45 down-regulated circRNAs in HCC tissues using RNA sequencing (P<0.05). Among these circRNAs, hsa_circ_0006834 was found to be significantly down-regulated in HCC tissues, and its expression level was negatively correlated with vascular invasion and Barcelona Clinic Liver Cancer (BCLC) stage and positively with recurrence-free survival and overall survival of the patients (P<0.05). As an independent prognostic factor for HCC, hsa_circ_0006834 had an AUC of 0.750 and 0.7601 for predicting 2-year recurrence-free survival and overall survival of HCC patients, respectively. Conclusion hsa_circ_0006834 is a new potential biomarker for evaluating the prognosis of HCC patients.

Key words: hepatocellular carcinoma; circular RNA; hsa_circ_0006834; prognosis; biomarker