Abstract: Objective To investigate the role of poly(ADP-ribose) polymerases-1 (PARP-1)-mediated blockade of autophagic flow in myocardial ischemia-reperfusion injury. Methods H9c2 cells, a rat cardiac myocyte line, were divided into control group, hypoxia/ reoxygenation model group (H/R group), PARP-1 inhibitor (PJ34) group, and PJ34 + H/R group. The total protein was extracted from the cells in each group to detect the expressions of pADPr, Bax, the DNA damage marker protein p- γH2ax, and autophagic flow-associated proteins LC3BII/LC3I, Beclin-1, and P62 using Western blotting. Results Compared with the control cells, the cells with H/R exhibited significantly increased expressions of pADPr, Bax and p-γH2ax (P<0.05). The expressions of LC3B II, beclin-1 and p62 were also increased significantly in the cells with H/R (P<0.05), indicating the block of the autophagic flow. The application of PARP-1 inhibitor PJ34 in the cells with H/R significantly inhibited the expressions of pADPr (P<0.05) and Bax (P<0.01), and alleviated DNA damage in the cells. PJ34 treatment did not cause significant changes in the expressions of LC3B II and beclin-1 but significantly decreased the expression of p62 (P<0.05) in the cells with H/R. Conclusion Block of autophagic flow mediated by PARP-1 activation plays a role in myocardial ischemiareperfusion injury, and inhibition of PARP-1 activity can reverse autophagic flow block to reduce the injury.