南方医科大学学报

南方医科大学学报 ›› 2018, Vol. 38 ›› Issue (08): 1020-.

• • 上一篇    

蜂胶对Triton-WR1339所致高脂血症小鼠的降脂作用及调控脂质代谢机制

黄晓其，吴晓丽，颜思珊，兰天   

  • 出版日期:2018-08-20 发布日期:2018-08-20

Lipid-lowering effect of propolis in mice with Triton-WR1339-induced hyperlipidemia and its mechanism for regulating lipid metabolism

  • Online:2018-08-20 Published:2018-08-20

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摘要： 目的探究蜂胶对Triton-WR1339所致高脂血症的降脂作用及调控脂质代谢机制。方法将C57BL/6小鼠随机分为7组， 10 只/组，雌雄各半，分别为正常组、模型组、非诺贝特组（30 mg/kg）、蜂胶HB01 高剂量组（60 mg/kg）、蜂胶HB01 低剂量组 （30 mg/kg）、蜂胶HB02高剂量组（60 mg/kg）和蜂胶HB02低剂量组（30 mg/kg）。采用肌肉注射Triton WR-1339试剂造成高脂 血症模型，灌胃1周后眼眶取血，离心后测定血清中总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL）、低密度脂蛋白 胆固醇（LDL）、丙二醛（MDA）、总超氧化物歧化酶（SOD）、谷丙转氨酶（GPT）、谷草转氨酶（GOT）等指标；取肝脏组织，置-80 ℃ 保存，Western blot法测定脂质转运蛋白的表达水平。结果与正常组相比，模型组血清中TC、TG、LDL、MDA、GPT、GOT的含 量升高，HDL含量降低、SOD活力下降（P<0.05）。与模型组比较，阳性药（非诺贝特：30 mg/kg）和两种蜂胶（高剂量组：60 mg/kg； 低剂量组：30 mg/kg）都能明显降低TC、TG、LDL、MDA、GPT、GOT含量而升高HDL含量，促进SOD活力，差异有统计学意义 （P<0.05），且蜂胶的效果略优于阳性药组。与正常组相比，Triton-WR1339 诱导的模型组肝脏中的ABCA1、ABCG8、低密度 脂蛋白和SR-B1 等脂质转运蛋白显著下降，差异有统计学意义（P<0.05），但阳性药（非诺贝特：30 mg/kg）和蜂胶（高剂量组： 60 mg/kg；低剂量组：30 mg/kg）干预后均能逆转这一下降趋势，蜂胶组的干预效果略优于阳性药组，差异有统计学意义（P<0.05）。 结论蜂胶具有显著的降血脂作用，其作用机制与改善肝脏脂质代谢紊乱、调控脂质代谢转运蛋白相关。

Abstract: Objective To evaluate the therapeutic effect of propolis against Triton-WR1339-induced hyperlipidemia in mice and explore the underlying mechanism. Methods C57BL/6 mice were randomly divided into 7 groups (n=10), including the control group, hyperlipidemia model group, fenofibrate (30 mg/kg) treatment group, and 4 treatment groups treated with low- (30 mg/kg) or high-dose (60 mg/kg) propolis HB01 or HB02. In all but the control group, acute hyperlipidemia models were established by intramuscular injection of Triton WR-1339, and corresponding treatments were administered via gastric lavage for 7 days. After the treatments, blood samples were collected for testing the levels of total cholesterol (TC), triglycerides (TG), highdensity lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), malondialdehyde (MDA), superoxide dismutase (SOD), alanine aminotransferase (GPT), and aspartate aminotransferase (GOT); Western blotting was used to detect the expressions of the proteins involved in lipid metabolism in the liver tissues including ABCA1, ABCG8, LDLR, and SR-B1. Results Compared with the normal control group, the mice with Triton-WR1339-induced hyperlipidemia showed significantly increased levels of TC, TG, LDL, MDA, GPT, and GOT and lowered HDL-C levels and SOD activity (P<0.05). Treatments with fenofibrate and the 2 propolis at either low or high dose significantly reversed Triton-WR1339-induced changes in blood lipids (P<0.05), and the effects of propolis were more potent. Triton-WR1339 injection also significantly decreased the expressions levels of ABCA1, ABCG8, LDLR, and SR-B1 in the liver (P<0.05), and these changes were obviously reversed by treatments with fenofibrate and propolis (P<0.05), especially by the latter. Conclusion The lipid-lowering effects of propolis are mediated by improving lipid metabolism and regulating the expressions of lipid transport proteins in the liver tissue.