南方医科大学学报

南方医科大学学报 ›› 2017, Vol. 37 ›› Issue (04): 444-.

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上皮性卵巢癌中八聚体结合转录因子4、Notch1及DLL4的表达及其临床意义

俞岚，焦云杰，周蕾，宋文庆，武世伍，王丹娜   

  • 出版日期:2017-04-20 发布日期:2017-04-20

Expressions of OCT4, Notch1 and DLL4 and their clinical implications in epithelial ovarian cancer

  • Online:2017-04-20 Published:2017-04-20

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摘要： 目的探讨上皮性卵巢癌（EOC）中八聚体结合转录因子4（OCT4）、Notch1及DLL4蛋白表达之间的关系及其功能意义。 方法收集207例EOC术后标本和65例卵巢良性上皮性肿瘤标本，应用免疫组化学法检测EOC和卵巢良性上皮性肿瘤组织中 OCT4、Notch1以及DLL4蛋白的表达情况。结果EOC组织和卵巢良性上皮性肿瘤组织中，OCT4、Notch1和DLL4蛋白的阳性 表达率分别为60.0%、61.8%、60.9%和9.2%、6.2%、0，差异均有统计学意义（P<0.05）；OCT4、Notch1和DLL4的表达与EOC的组 织学分化、FIGO分期、盆腔淋巴结转移有关（P<0.05）；DLL4蛋白的表达分别与OCT4和Notch1的表达呈正相关关系（r值分别 为0.758和0.704，P均<0.001），同时，OCT4与Notch1蛋白的表达亦呈正相关（r=0.645，P<0.001）。Kaplan-Meier生存分析表明， OCT4、Notch1和DLL4蛋白的过表达均与患者的生存率有关，3种蛋白表达阳性组患者生存率均明显低于蛋白表达阴性组患者 （P<0.05）。多因素分析：OCT4、DLL4 蛋白的表达和FIGO 分期是影响EOC根治术后患者预后的独立因素（P<0.05）。结论 OCT4、Notch1及DLL4的过表达与EOC的组织学分化程度、转移和预后等因素密切相关，联合检测这些指标可能对于EOC患 者的病情进展和预后判断给予重要提示。

Abstract: Objective To investigate the correlations among OCT4, Notch1 and DLL4 and their association with the clinicopathological features of patients with epithelial ovarian cancer (EOC). Methods A total of 207 specimens of EOC and 65 specimens of benign ovarian epithelial tumor tissues were examined for expressions of OCT4, Notch1 and DLL4 proteins using immunohistochemistry. Results The positivity rates of OCT4, Notch1 and DLL4 in EOC tissues were 60.0%, 61.8% and 60.9%, respectively, significantly higher than the rates in benign epithelial tumor tissues (9.2%, 6.2%, and 0, respectively; P< 0.05). The expressions of OCT4, Notch1 and DLL4 in EOC were significantly correlated with tumor differentiation, FIGO stage, and lymph node metastasis (P<0.05). DLL4 was positively correlated with OCT4 and Notch1 expressions (r=0.758 and 0.704, respectively, P<0.001), and the latter two were also positively correlated (r=0.645, P<0.001). Overexpressions of OCT4, Notch1 and DLL4 were associated with a poor prognosis, and the survival rate was significantly lower in patients positive for OCT4, Notch1, and DLL4 than in the negative patients (P<0.05). FIGO stage and expressions of OCT4 and DLL4 were independent prognostic factors of EOC (P<0.05). Conclusion The expressions of OCT4, Notch1 and DLL4 are correlated with the differentiation, lymph node metastasis, clinical stage and prognosis of EOC. Combined detection of the 3 proteins has an important value in predicting the progression and prognosis of EOC.