南方医科大学学报

南方医科大学学报 ›› 2016, Vol. 36 ›› Issue (02): 186-.

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慢性乙型肝炎患者PBMCs中ROG、GATA3和T-bet mRNA水平及其意义

张卡，舒欣，骆海飞，朱凤琴，曹红，徐启桓，李刚   

  • 出版日期:2016-02-20 发布日期:2016-02-20

ROG,GATA3 and T-bet mRNA levels in peripheral blood mononuclear cells in patients with chronic hepatitis B

  • Online:2016-02-20 Published:2016-02-20

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摘要： 目的探讨慢性乙型肝炎患者外周血单个核细胞（PBMC）中转录因子ROG、GATA3和T-bet mRNA水平变化及意义。方
法收集135例慢性乙型肝炎患者（轻度45例、中度42例、重度48例）和15例健康志愿者（正常对照组）的外周血，分离单个核细
胞，提取总RNA，用实时定量PCR的方法检测ROG、GATA3和T-bet mRNA水平。应用SPSS16.0软件进行统计分析。结果慢
性乙型肝炎轻度、中度组和重度组患者外周血PBMCs中T-bet mRNA表达水平均明显高于健康对照组人群，组内比较差异均具
有统计学意义（P<0.05）。慢性乙型肝炎重度组患者外周血PBMCs中ROG mRNA表达水平明显高于健康对照组人群、轻度组
和中度组患者，差异均有统计学意义（P<0.05）。而轻度组、中度组患者和健康对照组比较差异均无统计学意义（P>0.05）。慢性
乙型肝炎中度组和重度组患者外周血PBMCs中GATA3 mRNA表达水平明显高于健康对照组人群和轻度组患者，差异均具有
统计学意义（P<0.05）；而轻度组和健康对照、中度组患者和重度组比较差异均无统计学意义（P>0.05）。慢性乙型肝炎轻度、中
度组和重度组患者T-bet/GATA3比值明显高于健康对照组人群，差异均具有统计学意义（P<0.05）；但3组之间比较差异无统计
学意义（P>0.05）。ROG表达水平与GATA3和T-bet/GATA3比值均无相关性（P>0.05）。结论ROG、GATA3和T-bet 在慢性乙
型肝炎患者外周血PBMCs中表达水平上调，并参与疾病的发生和疾病进展。ROG在纠正和维持Th1/Th2的新平衡状态过程中
起重要作用。

Abstract: Objective To investigate the role of ROG, GATA3 and T-bet in the progression of chronic hepatitis B (CHB). Methods
The mRNA levels of ROG, GATA3 and T-bet in peripheral blood mononuclear cells (PBMCs) from 135 patients with CHB
(including 45 mild cases, 42 moderate cases, and 48 severe cases) and 15 healthy control subjects were detected by real-time
quantitative PCR. Results The levels of T-bet mRNA in the PBMCs were significantly higher in CHB patients than in the
healthy controls (P<0.05), and also differed significantly between the 3 groups of CHB patients (P<0.05). ROG mRNA levels
were significantly higher in severe cases of CHB than in the healthy controls and mild and moderate CHB cases (P<0.05), but
were similar among the latter 3 groups (P>0.05). The mRNA level of GATA3 in the PBMCs were significantly higher in
moderate and severe CHB cases than in the healthy controls and mild CHB cases (P<0.05). The T-bet/GATA3 ratio was
significantly greater in the 3 CHB groups than in the control group (P<0.05) but comparable between the 3 CHB groups (P>
0.05). ROG levels were not correlated with GATA3 levels or T-bet/GATA3 ratio in the CHB cases. Conclusions The mRNA
levels of ROG, GATA3 and T-bet in the PBMCs are obviously up-regulated in CHB patients and these 3 genes may participate
in the progression of CHB. ROG plays an important role in correcting and maintaining the new balance of Th1/Th2.