南方医科大学学报

南方医科大学学报 ›› 2015, Vol. 35 ›› Issue (12): 1802-.

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黄芪多糖干预树突状细胞基因表达与抗动脉粥样硬化的机制

陈朝俊，傅强，李玥珺，李志樑   

  • 出版日期:2015-12-20 发布日期:2015-12-20

Effect of Astragalus mongholicus polysaccharides on gene expression profiles of dendritic
cells isolated from healthy donors

  • Online:2015-12-20 Published:2015-12-20

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摘要： 目的研究黄芪多糖（APS）对人外周血单核细胞（PBMC）源性树突状细胞（DCs）的基因表达和其功能变化的影响，进一步
探讨黄芪多糖的抗AS的作用机制。方法以健康人外周血分离PBMC源性DCs和血清作为研究对象，培育5 d后，随机分为
APS组和对照组。其中APS组给予200 mg/L APS孵育过夜，对照组不给干预。通过基因芯片和RT-PCR技术，观察APS处理
PBMC源性DCs的免疫功能和其他基因表达的差异与AS发生发展的关系。结果与对照组相比，APS组CD36（0.97±0.23 vs
5.45±1.14）、IL-27（1.08±0.22 vs 2.97±0.61）基因表达相对量显著性上调；APS组IFI16（0.98±0.18 vs 0.46±0.11）基因表达相对量
显著性下调。结论适量的APS可以上调DCs膜表面与抗原递呈相关的CD36、IL-27的表达，下调IFI16的表达，对增加DCs的
免疫活性，促进DCs的成熟与分化有显著的影响。APS对AS的发生发展具有明显的积极地干预作用，有着重要的积极地临床
意义。

Abstract: Objective To investigate the anti-atherosclerosis mechanism of Astragalus mongholicus polysaccharides (APS) by
examining its effect on gene expression profiles of the dendritic cells (DCs) from healthy donors. Methods Peripheral blood
DCs from healthy donors were incubated with 200 mg/L APS overnight, and changes in the gene expression profiles were
investigated using microarray technique and RT-PCR. Result Compared with the control cells, APS-treated DCs showed
significantly up-regulated expressions of CD36 (0.97±0.23 vs 5.45±1.14) and IL-27 (1.08±0.22 vs 2.97±0.61) and down-regulated
expression of expression of IFI16 (0.98±0.18 vs 0.46±0.11). Conclusion APS can promote the maturation and differentiation of
DCs by up-regulating CD36 and IL-27 and down-regulating IFI16, and thus positively affects the occurrence and progression
of the atherosclerosis.