Abstract: Objective To explore the relationship between the occurrence of sub-health and health promotion lifestyle in
Guangdong province to provide epidemiological evidences for prevention of sub-health. Methods A 1:1 nested case-control
study was carried out involving 133 cases from the cohort study paired with 133 control case matched for gender and age.
Results The univariate conditional logistic regression model showed no significant association of marital status, education
level, or body mass index with the occurrence of sub-health, but a significant correlation was found between lifestyle and
sub-health with an odd ratio of 5.90 (95% CI: 3.12-11.16, P=0.000). Analysis of the multivariate conditional logistic regression
model on the 6 subscales of health promotion lifestyle showed that stress management was significantly correlated with the
occurrence of sub-health with an odd ratio of 4.00 (95% CI: 1.54-10.40, P=0.004). Conclusion A unhealthy lifestyle, especially in
terms of stress management, is the major risk factor contributing to the occurrence of sub-health.